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Aureobasidiumcomprises dimorphic yeast-like fungi that usually produce melanised cells at maturity. Species are globally distributed and ubiquitous, colonizing a variety of habitats. At present, ca 40 species are accepted, with the type,A. pullulans, representing a complex of unresolved cryptic species. In this study, we isolated 128Aureobasidiumfrom multiple temperate and tropical regions. We performed multigene analyses using eight loci (ITS, 28S,EF1a,ELO2,RPB2,BTUB, mtLSU and mtSSU) on new isolates and including sequences from type material for all availableAureobasidiumspecies. Data on growth, physiological profiles, micro- and macromorphological attributes were also collected and analysed. Several DNA-based species delimitation methods were evaluated for their ability to delimit species. We found that assimilation of D-quinic acid, L-sorbose, D-mannitol, gluconolactone, erythritol, L-arabinose, and sodium succinate dibasic hexahydrate were important in delineating species ofAureobasidiumand note that production of pigmentation in culture often takes longer than the 14 d standard for carbon assimilations. Genealogical concordance phylogenetic species recognition criteria (GCPSR) provided the most consistent results for species delimitation. We describe nine new species ofAureobasidium (A. albui,A. cavalettoi,A. diazvalderramae,A. ellingtonae,A. essambei,A. peruvianum,A. rubi,A. toomeae, andA. vanuatuense), make new combinations forA. aubasidani(≡ A. pullulansvar.aubasidani),A. fermentans(≡ Pullularia fermentans), andA. mahoniae(≡ Selenophoma mahoniae), validate and provide descriptions forA. mustiandA. uvarum, and provide lecto- and epitypes forDematium pullulans,the basionym ofA. pullulans. Finally, we resolved the phylogeny forAureobasidium, reduceKabatiella(based onK. microsticta) to synonymy, and provide an updated list of species to facilitate future studies.